A team of researchers from the USA and Taiwan have developed a low-cost biosensor for the foodborne pathogen Listeria monocytogenes using a novel nanotechnology-based method.
The team, from the University of Maine (Orono), National Chio Tung University and the Apex Biotechnology Corp., modified screen-printed carbon electrode strips, used for monitoring glucose in diabetic patients, with gold nanoparticles and antibodies specific to L. monocytogenes to create the sensors. Detection was amplified by conjugating secondary enzyme-labelled antibodies to the gold nanoparticles.
The resulting biosensor is said to be inexpensive and capable of detecting Listeria cells at levels of 2 log CFU/g in wild blueberry samples. The researchers also suggest that the technique could form the basis of a commercial rapid detection method for foodborne pathogens.
The development of the biosensor is reported in the journal Industrial Biotechnology and can be found in full here.